Virus:Article Title: Evolution of Mycobacterium tuberculosis transcription regulation is associated with increased transmission and drug resistance.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies CFP-10 polyclonal antibody Invitrogen CAT#PA5-122324; RRID: AB_2915896 GroEL2 BEI CAT#NR-13566 Mpt64 Invitrogen CAT#PA5-117508; RRID: AB_2902137 IRDye 680LT Donkey anti-Rabbit IgG Secondary LI-COR CAT#926-68023; RRID: AB_10706167 IRDye 800CW Goat anti-Mouse IgG Secondary LI-COR CAT#926-32210; RRID: AB_621842 Bacterial and virus strains NEB 5-alpha Competent E. coli NEB CAT#C2987I BL21-CodonPlus (DE3)-RP Competent Cells Agilent CAT#230255 Biological samples Mtb strains from Vietnam Holt et al. 13 N/A Mtb strains from Peru Luo et al. 14 N/A Chemicals, peptides, and recombinant proteins 7H9 Media Fisher CAT#271310 OADC BD/Fisher CAT#212351 Taq NEB CAT#M0273S thermostable FEN1 NEB CAT#M0645S proteinase K NEB CAT#P8111S iTaq qPCR master mix Bio-Rad CAT1725120 Maxima H Minus Reverse Transcriptase ThermoFisher CAT#EP0753 exonuclease I NEB CAT#M0293S RNase H NEB CAT#M0297S T4 Ligase (high concentration) NEB CAT#M0202L KAPA HiFi Master Mix Roche CAT#7958927001 Roche cOmplete Protease Inhibitor Cocktail Millipore Sigma CAT#11697498001 Ni-NTA Agarose Qiagen CAT#30210 Talon resin Clontech CAT#635501 Sera-Mag Speed Beads Cytiva CAT#65152105050250 6X Laemmli SDS-Sample Buffer Boston BioProducts CAT#BP-111NR-25ml SeeBlue Plus2 Pre-stained Protein Standard Invitrogen CAT#LC5925 NuPAGE 4 to 12%, Bis-Tris Invitrogen CAT#NP0322BOX NuPAGE MES SDS Running Buffer Invitrogen CAT#NP0002 10X Transfer Buffer Boston BioProducts CAT#BP-194-1L Blocking Buffer for Fluorescent Western Blotting Rockland CAT#MB-070 Critical commercial assays NEBNext Ultra II DNA Library Prep Kit for Illumina NEB CAT#E7645S Qubit Protein Assay Invitrogen CAT#Q33211 Slide-A-Lyzer G3 Dialysis Cassette Thermo Scientific CAT#A52971 (Continued on next page) ll OPEN ACCESS Cell 188, 1–16.e1–e8, November 13, 2025 e1 Please cite this article in press as: Culviner et al., Evolution of Mycobacterium tuberculosis transcription regulation is associated with increased transmission and drug resistance, Cell (2025), https://doi.org/10.1016/j.cell.2025.09.005 Article ..
Recombinant:Article Title: Evolution of Mycobacterium tuberculosis transcription regulation is associated with increased transmission and drug resistance.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies CFP-10 polyclonal antibody Invitrogen CAT#PA5-122324; RRID: AB_2915896 GroEL2 BEI CAT#NR-13566 Mpt64 Invitrogen CAT#PA5-117508; RRID: AB_2902137 IRDye 680LT Donkey anti-Rabbit IgG Secondary LI-COR CAT#926-68023; RRID: AB_10706167 IRDye 800CW Goat anti-Mouse IgG Secondary LI-COR CAT#926-32210; RRID: AB_621842 Bacterial and virus strains NEB 5-alpha Competent E. coli NEB CAT#C2987I BL21-CodonPlus (DE3)-RP Competent Cells Agilent CAT#230255 Biological samples Mtb strains from Vietnam Holt et al. 13 N/A Mtb strains from Peru Luo et al. 14 N/A Chemicals, peptides, and recombinant proteins 7H9 Media Fisher CAT#271310 OADC BD/Fisher CAT#212351 Taq NEB CAT#M0273S thermostable FEN1 NEB CAT#M0645S proteinase K NEB CAT#P8111S iTaq qPCR master mix Bio-Rad CAT1725120 Maxima H Minus Reverse Transcriptase ThermoFisher CAT#EP0753 exonuclease I NEB CAT#M0293S RNase H NEB CAT#M0297S T4 Ligase (high concentration) NEB CAT#M0202L KAPA HiFi Master Mix Roche CAT#7958927001 Roche cOmplete Protease Inhibitor Cocktail Millipore Sigma CAT#11697498001 Ni-NTA Agarose Qiagen CAT#30210 Talon resin Clontech CAT#635501 Sera-Mag Speed Beads Cytiva CAT#65152105050250 6X Laemmli SDS-Sample Buffer Boston BioProducts CAT#BP-111NR-25ml SeeBlue Plus2 Pre-stained Protein Standard Invitrogen CAT#LC5925 NuPAGE 4 to 12%, Bis-Tris Invitrogen CAT#NP0322BOX NuPAGE MES SDS Running Buffer Invitrogen CAT#NP0002 10X Transfer Buffer Boston BioProducts CAT#BP-194-1L Blocking Buffer for Fluorescent Western Blotting Rockland CAT#MB-070 Critical commercial assays NEBNext Ultra II DNA Library Prep Kit for Illumina NEB CAT#E7645S Qubit Protein Assay Invitrogen CAT#Q33211 Slide-A-Lyzer G3 Dialysis Cassette Thermo Scientific CAT#A52971 (Continued on next page) ll OPEN ACCESS Cell 188, 1–16.e1–e8, November 13, 2025 e1 Please cite this article in press as: Culviner et al., Evolution of Mycobacterium tuberculosis transcription regulation is associated with increased transmission and drug resistance, Cell (2025), https://doi.org/10.1016/j.cell.2025.09.005 Article ..
Real-time Polymerase Chain Reaction:Article Title: Evolution of Mycobacterium tuberculosis transcription regulation is associated with increased transmission and drug resistance.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies CFP-10 polyclonal antibody Invitrogen CAT#PA5-122324; RRID: AB_2915896 GroEL2 BEI CAT#NR-13566 Mpt64 Invitrogen CAT#PA5-117508; RRID: AB_2902137 IRDye 680LT Donkey anti-Rabbit IgG Secondary LI-COR CAT#926-68023; RRID: AB_10706167 IRDye 800CW Goat anti-Mouse IgG Secondary LI-COR CAT#926-32210; RRID: AB_621842 Bacterial and virus strains NEB 5-alpha Competent E. coli NEB CAT#C2987I BL21-CodonPlus (DE3)-RP Competent Cells Agilent CAT#230255 Biological samples Mtb strains from Vietnam Holt et al. 13 N/A Mtb strains from Peru Luo et al. 14 N/A Chemicals, peptides, and recombinant proteins 7H9 Media Fisher CAT#271310 OADC BD/Fisher CAT#212351 Taq NEB CAT#M0273S thermostable FEN1 NEB CAT#M0645S proteinase K NEB CAT#P8111S iTaq qPCR master mix Bio-Rad CAT1725120 Maxima H Minus Reverse Transcriptase ThermoFisher CAT#EP0753 exonuclease I NEB CAT#M0293S RNase H NEB CAT#M0297S T4 Ligase (high concentration) NEB CAT#M0202L KAPA HiFi Master Mix Roche CAT#7958927001 Roche cOmplete Protease Inhibitor Cocktail Millipore Sigma CAT#11697498001 Ni-NTA Agarose Qiagen CAT#30210 Talon resin Clontech CAT#635501 Sera-Mag Speed Beads Cytiva CAT#65152105050250 6X Laemmli SDS-Sample Buffer Boston BioProducts CAT#BP-111NR-25ml SeeBlue Plus2 Pre-stained Protein Standard Invitrogen CAT#LC5925 NuPAGE 4 to 12%, Bis-Tris Invitrogen CAT#NP0322BOX NuPAGE MES SDS Running Buffer Invitrogen CAT#NP0002 10X Transfer Buffer Boston BioProducts CAT#BP-194-1L Blocking Buffer for Fluorescent Western Blotting Rockland CAT#MB-070 Critical commercial assays NEBNext Ultra II DNA Library Prep Kit for Illumina NEB CAT#E7645S Qubit Protein Assay Invitrogen CAT#Q33211 Slide-A-Lyzer G3 Dialysis Cassette Thermo Scientific CAT#A52971 (Continued on next page) ll OPEN ACCESS Cell 188, 1–16.e1–e8, November 13, 2025 e1 Please cite this article in press as: Culviner et al., Evolution of Mycobacterium tuberculosis transcription regulation is associated with increased transmission and drug resistance, Cell (2025), https://doi.org/10.1016/j.cell.2025.09.005 Article ..
Reverse Transcription:Article Title: Evolution of Mycobacterium tuberculosis transcription regulation is associated with increased transmission and drug resistance.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies CFP-10 polyclonal antibody Invitrogen CAT#PA5-122324; RRID: AB_2915896 GroEL2 BEI CAT#NR-13566 Mpt64 Invitrogen CAT#PA5-117508; RRID: AB_2902137 IRDye 680LT Donkey anti-Rabbit IgG Secondary LI-COR CAT#926-68023; RRID: AB_10706167 IRDye 800CW Goat anti-Mouse IgG Secondary LI-COR CAT#926-32210; RRID: AB_621842 Bacterial and virus strains NEB 5-alpha Competent E. coli NEB CAT#C2987I BL21-CodonPlus (DE3)-RP Competent Cells Agilent CAT#230255 Biological samples Mtb strains from Vietnam Holt et al. 13 N/A Mtb strains from Peru Luo et al. 14 N/A Chemicals, peptides, and recombinant proteins 7H9 Media Fisher CAT#271310 OADC BD/Fisher CAT#212351 Taq NEB CAT#M0273S thermostable FEN1 NEB CAT#M0645S proteinase K NEB CAT#P8111S iTaq qPCR master mix Bio-Rad CAT1725120 Maxima H Minus Reverse Transcriptase ThermoFisher CAT#EP0753 exonuclease I NEB CAT#M0293S RNase H NEB CAT#M0297S T4 Ligase (high concentration) NEB CAT#M0202L KAPA HiFi Master Mix Roche CAT#7958927001 Roche cOmplete Protease Inhibitor Cocktail Millipore Sigma CAT#11697498001 Ni-NTA Agarose Qiagen CAT#30210 Talon resin Clontech CAT#635501 Sera-Mag Speed Beads Cytiva CAT#65152105050250 6X Laemmli SDS-Sample Buffer Boston BioProducts CAT#BP-111NR-25ml SeeBlue Plus2 Pre-stained Protein Standard Invitrogen CAT#LC5925 NuPAGE 4 to 12%, Bis-Tris Invitrogen CAT#NP0322BOX NuPAGE MES SDS Running Buffer Invitrogen CAT#NP0002 10X Transfer Buffer Boston BioProducts CAT#BP-194-1L Blocking Buffer for Fluorescent Western Blotting Rockland CAT#MB-070 Critical commercial assays NEBNext Ultra II DNA Library Prep Kit for Illumina NEB CAT#E7645S Qubit Protein Assay Invitrogen CAT#Q33211 Slide-A-Lyzer G3 Dialysis Cassette Thermo Scientific CAT#A52971 (Continued on next page) ll OPEN ACCESS Cell 188, 1–16.e1–e8, November 13, 2025 e1 Please cite this article in press as: Culviner et al., Evolution of Mycobacterium tuberculosis transcription regulation is associated with increased transmission and drug resistance, Cell (2025), https://doi.org/10.1016/j.cell.2025.09.005 Article ..
Concentration Assay:Article Title: Evolution of Mycobacterium tuberculosis transcription regulation is associated with increased transmission and drug resistance.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies CFP-10 polyclonal antibody Invitrogen CAT#PA5-122324; RRID: AB_2915896 GroEL2 BEI CAT#NR-13566 Mpt64 Invitrogen CAT#PA5-117508; RRID: AB_2902137 IRDye 680LT Donkey anti-Rabbit IgG Secondary LI-COR CAT#926-68023; RRID: AB_10706167 IRDye 800CW Goat anti-Mouse IgG Secondary LI-COR CAT#926-32210; RRID: AB_621842 Bacterial and virus strains NEB 5-alpha Competent E. coli NEB CAT#C2987I BL21-CodonPlus (DE3)-RP Competent Cells Agilent CAT#230255 Biological samples Mtb strains from Vietnam Holt et al. 13 N/A Mtb strains from Peru Luo et al. 14 N/A Chemicals, peptides, and recombinant proteins 7H9 Media Fisher CAT#271310 OADC BD/Fisher CAT#212351 Taq NEB CAT#M0273S thermostable FEN1 NEB CAT#M0645S proteinase K NEB CAT#P8111S iTaq qPCR master mix Bio-Rad CAT1725120 Maxima H Minus Reverse Transcriptase ThermoFisher CAT#EP0753 exonuclease I NEB CAT#M0293S RNase H NEB CAT#M0297S T4 Ligase (high concentration) NEB CAT#M0202L KAPA HiFi Master Mix Roche CAT#7958927001 Roche cOmplete Protease Inhibitor Cocktail Millipore Sigma CAT#11697498001 Ni-NTA Agarose Qiagen CAT#30210 Talon resin Clontech CAT#635501 Sera-Mag Speed Beads Cytiva CAT#65152105050250 6X Laemmli SDS-Sample Buffer Boston BioProducts CAT#BP-111NR-25ml SeeBlue Plus2 Pre-stained Protein Standard Invitrogen CAT#LC5925 NuPAGE 4 to 12%, Bis-Tris Invitrogen CAT#NP0322BOX NuPAGE MES SDS Running Buffer Invitrogen CAT#NP0002 10X Transfer Buffer Boston BioProducts CAT#BP-194-1L Blocking Buffer for Fluorescent Western Blotting Rockland CAT#MB-070 Critical commercial assays NEBNext Ultra II DNA Library Prep Kit for Illumina NEB CAT#E7645S Qubit Protein Assay Invitrogen CAT#Q33211 Slide-A-Lyzer G3 Dialysis Cassette Thermo Scientific CAT#A52971 (Continued on next page) ll OPEN ACCESS Cell 188, 1–16.e1–e8, November 13, 2025 e1 Please cite this article in press as: Culviner et al., Evolution of Mycobacterium tuberculosis transcription regulation is associated with increased transmission and drug resistance, Cell (2025), https://doi.org/10.1016/j.cell.2025.09.005 Article ..
Protease Inhibitor:Article Title: Evolution of Mycobacterium tuberculosis transcription regulation is associated with increased transmission and drug resistance.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies CFP-10 polyclonal antibody Invitrogen CAT#PA5-122324; RRID: AB_2915896 GroEL2 BEI CAT#NR-13566 Mpt64 Invitrogen CAT#PA5-117508; RRID: AB_2902137 IRDye 680LT Donkey anti-Rabbit IgG Secondary LI-COR CAT#926-68023; RRID: AB_10706167 IRDye 800CW Goat anti-Mouse IgG Secondary LI-COR CAT#926-32210; RRID: AB_621842 Bacterial and virus strains NEB 5-alpha Competent E. coli NEB CAT#C2987I BL21-CodonPlus (DE3)-RP Competent Cells Agilent CAT#230255 Biological samples Mtb strains from Vietnam Holt et al. 13 N/A Mtb strains from Peru Luo et al. 14 N/A Chemicals, peptides, and recombinant proteins 7H9 Media Fisher CAT#271310 OADC BD/Fisher CAT#212351 Taq NEB CAT#M0273S thermostable FEN1 NEB CAT#M0645S proteinase K NEB CAT#P8111S iTaq qPCR master mix Bio-Rad CAT1725120 Maxima H Minus Reverse Transcriptase ThermoFisher CAT#EP0753 exonuclease I NEB CAT#M0293S RNase H NEB CAT#M0297S T4 Ligase (high concentration) NEB CAT#M0202L KAPA HiFi Master Mix Roche CAT#7958927001 Roche cOmplete Protease Inhibitor Cocktail Millipore Sigma CAT#11697498001 Ni-NTA Agarose Qiagen CAT#30210 Talon resin Clontech CAT#635501 Sera-Mag Speed Beads Cytiva CAT#65152105050250 6X Laemmli SDS-Sample Buffer Boston BioProducts CAT#BP-111NR-25ml SeeBlue Plus2 Pre-stained Protein Standard Invitrogen CAT#LC5925 NuPAGE 4 to 12%, Bis-Tris Invitrogen CAT#NP0322BOX NuPAGE MES SDS Running Buffer Invitrogen CAT#NP0002 10X Transfer Buffer Boston BioProducts CAT#BP-194-1L Blocking Buffer for Fluorescent Western Blotting Rockland CAT#MB-070 Critical commercial assays NEBNext Ultra II DNA Library Prep Kit for Illumina NEB CAT#E7645S Qubit Protein Assay Invitrogen CAT#Q33211 Slide-A-Lyzer G3 Dialysis Cassette Thermo Scientific CAT#A52971 (Continued on next page) ll OPEN ACCESS Cell 188, 1–16.e1–e8, November 13, 2025 e1 Please cite this article in press as: Culviner et al., Evolution of Mycobacterium tuberculosis transcription regulation is associated with increased transmission and drug resistance, Cell (2025), https://doi.org/10.1016/j.cell.2025.09.005 Article ..
Blocking Assay:Article Title: Evolution of Mycobacterium tuberculosis transcription regulation is associated with increased transmission and drug resistance.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies CFP-10 polyclonal antibody Invitrogen CAT#PA5-122324; RRID: AB_2915896 GroEL2 BEI CAT#NR-13566 Mpt64 Invitrogen CAT#PA5-117508; RRID: AB_2902137 IRDye 680LT Donkey anti-Rabbit IgG Secondary LI-COR CAT#926-68023; RRID: AB_10706167 IRDye 800CW Goat anti-Mouse IgG Secondary LI-COR CAT#926-32210; RRID: AB_621842 Bacterial and virus strains NEB 5-alpha Competent E. coli NEB CAT#C2987I BL21-CodonPlus (DE3)-RP Competent Cells Agilent CAT#230255 Biological samples Mtb strains from Vietnam Holt et al. 13 N/A Mtb strains from Peru Luo et al. 14 N/A Chemicals, peptides, and recombinant proteins 7H9 Media Fisher CAT#271310 OADC BD/Fisher CAT#212351 Taq NEB CAT#M0273S thermostable FEN1 NEB CAT#M0645S proteinase K NEB CAT#P8111S iTaq qPCR master mix Bio-Rad CAT1725120 Maxima H Minus Reverse Transcriptase ThermoFisher CAT#EP0753 exonuclease I NEB CAT#M0293S RNase H NEB CAT#M0297S T4 Ligase (high concentration) NEB CAT#M0202L KAPA HiFi Master Mix Roche CAT#7958927001 Roche cOmplete Protease Inhibitor Cocktail Millipore Sigma CAT#11697498001 Ni-NTA Agarose Qiagen CAT#30210 Talon resin Clontech CAT#635501 Sera-Mag Speed Beads Cytiva CAT#65152105050250 6X Laemmli SDS-Sample Buffer Boston BioProducts CAT#BP-111NR-25ml SeeBlue Plus2 Pre-stained Protein Standard Invitrogen CAT#LC5925 NuPAGE 4 to 12%, Bis-Tris Invitrogen CAT#NP0322BOX NuPAGE MES SDS Running Buffer Invitrogen CAT#NP0002 10X Transfer Buffer Boston BioProducts CAT#BP-194-1L Blocking Buffer for Fluorescent Western Blotting Rockland CAT#MB-070 Critical commercial assays NEBNext Ultra II DNA Library Prep Kit for Illumina NEB CAT#E7645S Qubit Protein Assay Invitrogen CAT#Q33211 Slide-A-Lyzer G3 Dialysis Cassette Thermo Scientific CAT#A52971 (Continued on next page) ll OPEN ACCESS Cell 188, 1–16.e1–e8, November 13, 2025 e1 Please cite this article in press as: Culviner et al., Evolution of Mycobacterium tuberculosis transcription regulation is associated with increased transmission and drug resistance, Cell (2025), https://doi.org/10.1016/j.cell.2025.09.005 Article ..
Western Blot:Article Title: Evolution of Mycobacterium tuberculosis transcription regulation is associated with increased transmission and drug resistance.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies CFP-10 polyclonal antibody Invitrogen CAT#PA5-122324; RRID: AB_2915896 GroEL2 BEI CAT#NR-13566 Mpt64 Invitrogen CAT#PA5-117508; RRID: AB_2902137 IRDye 680LT Donkey anti-Rabbit IgG Secondary LI-COR CAT#926-68023; RRID: AB_10706167 IRDye 800CW Goat anti-Mouse IgG Secondary LI-COR CAT#926-32210; RRID: AB_621842 Bacterial and virus strains NEB 5-alpha Competent E. coli NEB CAT#C2987I BL21-CodonPlus (DE3)-RP Competent Cells Agilent CAT#230255 Biological samples Mtb strains from Vietnam Holt et al. 13 N/A Mtb strains from Peru Luo et al. 14 N/A Chemicals, peptides, and recombinant proteins 7H9 Media Fisher CAT#271310 OADC BD/Fisher CAT#212351 Taq NEB CAT#M0273S thermostable FEN1 NEB CAT#M0645S proteinase K NEB CAT#P8111S iTaq qPCR master mix Bio-Rad CAT1725120 Maxima H Minus Reverse Transcriptase ThermoFisher CAT#EP0753 exonuclease I NEB CAT#M0293S RNase H NEB CAT#M0297S T4 Ligase (high concentration) NEB CAT#M0202L KAPA HiFi Master Mix Roche CAT#7958927001 Roche cOmplete Protease Inhibitor Cocktail Millipore Sigma CAT#11697498001 Ni-NTA Agarose Qiagen CAT#30210 Talon resin Clontech CAT#635501 Sera-Mag Speed Beads Cytiva CAT#65152105050250 6X Laemmli SDS-Sample Buffer Boston BioProducts CAT#BP-111NR-25ml SeeBlue Plus2 Pre-stained Protein Standard Invitrogen CAT#LC5925 NuPAGE 4 to 12%, Bis-Tris Invitrogen CAT#NP0322BOX NuPAGE MES SDS Running Buffer Invitrogen CAT#NP0002 10X Transfer Buffer Boston BioProducts CAT#BP-194-1L Blocking Buffer for Fluorescent Western Blotting Rockland CAT#MB-070 Critical commercial assays NEBNext Ultra II DNA Library Prep Kit for Illumina NEB CAT#E7645S Qubit Protein Assay Invitrogen CAT#Q33211 Slide-A-Lyzer G3 Dialysis Cassette Thermo Scientific CAT#A52971 (Continued on next page) ll OPEN ACCESS Cell 188, 1–16.e1–e8, November 13, 2025 e1 Please cite this article in press as: Culviner et al., Evolution of Mycobacterium tuberculosis transcription regulation is associated with increased transmission and drug resistance, Cell (2025), https://doi.org/10.1016/j.cell.2025.09.005 Article ..
Qubit Protein Assay:Article Title: Evolution of Mycobacterium tuberculosis transcription regulation is associated with increased transmission and drug resistance.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies CFP-10 polyclonal antibody Invitrogen CAT#PA5-122324; RRID: AB_2915896 GroEL2 BEI CAT#NR-13566 Mpt64 Invitrogen CAT#PA5-117508; RRID: AB_2902137 IRDye 680LT Donkey anti-Rabbit IgG Secondary LI-COR CAT#926-68023; RRID: AB_10706167 IRDye 800CW Goat anti-Mouse IgG Secondary LI-COR CAT#926-32210; RRID: AB_621842 Bacterial and virus strains NEB 5-alpha Competent E. coli NEB CAT#C2987I BL21-CodonPlus (DE3)-RP Competent Cells Agilent CAT#230255 Biological samples Mtb strains from Vietnam Holt et al. 13 N/A Mtb strains from Peru Luo et al. 14 N/A Chemicals, peptides, and recombinant proteins 7H9 Media Fisher CAT#271310 OADC BD/Fisher CAT#212351 Taq NEB CAT#M0273S thermostable FEN1 NEB CAT#M0645S proteinase K NEB CAT#P8111S iTaq qPCR master mix Bio-Rad CAT1725120 Maxima H Minus Reverse Transcriptase ThermoFisher CAT#EP0753 exonuclease I NEB CAT#M0293S RNase H NEB CAT#M0297S T4 Ligase (high concentration) NEB CAT#M0202L KAPA HiFi Master Mix Roche CAT#7958927001 Roche cOmplete Protease Inhibitor Cocktail Millipore Sigma CAT#11697498001 Ni-NTA Agarose Qiagen CAT#30210 Talon resin Clontech CAT#635501 Sera-Mag Speed Beads Cytiva CAT#65152105050250 6X Laemmli SDS-Sample Buffer Boston BioProducts CAT#BP-111NR-25ml SeeBlue Plus2 Pre-stained Protein Standard Invitrogen CAT#LC5925 NuPAGE 4 to 12%, Bis-Tris Invitrogen CAT#NP0322BOX NuPAGE MES SDS Running Buffer Invitrogen CAT#NP0002 10X Transfer Buffer Boston BioProducts CAT#BP-194-1L Blocking Buffer for Fluorescent Western Blotting Rockland CAT#MB-070 Critical commercial assays NEBNext Ultra II DNA Library Prep Kit for Illumina NEB CAT#E7645S Qubit Protein Assay Invitrogen CAT#Q33211 Slide-A-Lyzer G3 Dialysis Cassette Thermo Scientific CAT#A52971 (Continued on next page) ll OPEN ACCESS Cell 188, 1–16.e1–e8, November 13, 2025 e1 Please cite this article in press as: Culviner et al., Evolution of Mycobacterium tuberculosis transcription regulation is associated with increased transmission and drug resistance, Cell (2025), https://doi.org/10.1016/j.cell.2025.09.005 Article ..
Transmission Assay:Article Title: Evolution of Mycobacterium tuberculosis transcription regulation is associated with increased transmission and drug resistance.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies CFP-10 polyclonal antibody Invitrogen CAT#PA5-122324; RRID: AB_2915896 GroEL2 BEI CAT#NR-13566 Mpt64 Invitrogen CAT#PA5-117508; RRID: AB_2902137 IRDye 680LT Donkey anti-Rabbit IgG Secondary LI-COR CAT#926-68023; RRID: AB_10706167 IRDye 800CW Goat anti-Mouse IgG Secondary LI-COR CAT#926-32210; RRID: AB_621842 Bacterial and virus strains NEB 5-alpha Competent E. coli NEB CAT#C2987I BL21-CodonPlus (DE3)-RP Competent Cells Agilent CAT#230255 Biological samples Mtb strains from Vietnam Holt et al. 13 N/A Mtb strains from Peru Luo et al. 14 N/A Chemicals, peptides, and recombinant proteins 7H9 Media Fisher CAT#271310 OADC BD/Fisher CAT#212351 Taq NEB CAT#M0273S thermostable FEN1 NEB CAT#M0645S proteinase K NEB CAT#P8111S iTaq qPCR master mix Bio-Rad CAT1725120 Maxima H Minus Reverse Transcriptase ThermoFisher CAT#EP0753 exonuclease I NEB CAT#M0293S RNase H NEB CAT#M0297S T4 Ligase (high concentration) NEB CAT#M0202L KAPA HiFi Master Mix Roche CAT#7958927001 Roche cOmplete Protease Inhibitor Cocktail Millipore Sigma CAT#11697498001 Ni-NTA Agarose Qiagen CAT#30210 Talon resin Clontech CAT#635501 Sera-Mag Speed Beads Cytiva CAT#65152105050250 6X Laemmli SDS-Sample Buffer Boston BioProducts CAT#BP-111NR-25ml SeeBlue Plus2 Pre-stained Protein Standard Invitrogen CAT#LC5925 NuPAGE 4 to 12%, Bis-Tris Invitrogen CAT#NP0322BOX NuPAGE MES SDS Running Buffer Invitrogen CAT#NP0002 10X Transfer Buffer Boston BioProducts CAT#BP-194-1L Blocking Buffer for Fluorescent Western Blotting Rockland CAT#MB-070 Critical commercial assays NEBNext Ultra II DNA Library Prep Kit for Illumina NEB CAT#E7645S Qubit Protein Assay Invitrogen CAT#Q33211 Slide-A-Lyzer G3 Dialysis Cassette Thermo Scientific CAT#A52971 (Continued on next page) ll OPEN ACCESS Cell 188, 1–16.e1–e8, November 13, 2025 e1 Please cite this article in press as: Culviner et al., Evolution of Mycobacterium tuberculosis transcription regulation is associated with increased transmission and drug resistance, Cell (2025), https://doi.org/10.1016/j.cell.2025.09.005 Article ..
|
Bio-Rad is a verified supplier 
Bio-Rad manufactures this product 